Fig. 1: IFT motility in VashL cells is frequently interrupted by stoppages. | Nature Communications

Fig. 1: IFT motility in VashL cells is frequently interrupted by stoppages.

From: Tubulin tyrosination/detyrosination regulate the affinity and sorting of intraflagellar transport trains on axonemal microtubule doublets

Fig. 1

A Kymographs of wild-type (WT, top) or VashL (bottom) IFT trains. In VashL cells, anterograde or retrograde train stoppages (green or magenta arrowheads respectively) often occur after crossing events (white arrowheads, see Fig. S3 for additional examples). B Violin plots of interuptions during runs in wild-type (grey plots) or VashL (blue plots) trains. Each datapoint represents an individual train. Horizontal line represents median of each dataset. Total number of trains analyzed in each case as shown. Pooled data from 20-30 flagella, 2-3 independent experiments. Statistics by Two-sided Wilcoxon Signed rank test. p-values as shown. C Violin plots of train injection rates from base or tip in wild-type (grey plots) or VashL (blue plots) cilia, respectively. Horizontal line represents median of each dataset. Total flagella analysed in each case as shown, 2–3 independent acquisitions. Statistics by Student’s two-tailed t-test. p-values as shown. D Kinetics of cilia recovery in wild-type (grey plot) or VashL (blue plot) cells, expressed as % w.r.t. cilia length pre-deciliation. Error bands, datapoints and dashed lines represent 95% CI, measured averages and logistical curve fits respectively. N = 12–35 cells for each time point, pooled data from 2 independent replicates for each case.

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