Fig. 7: TNC-Hippo signaling is involved in trauma-induced HO formation. | Nature Communications

Fig. 7: TNC-Hippo signaling is involved in trauma-induced HO formation.

From: A CD26+ tendon stem progenitor cell population contributes to tendon repair and heterotopic ossification

Fig. 7: TNC-Hippo signaling is involved in trauma-induced HO formation.The alternative text for this image may have been generated using AI.

a Scheme for establishment and tendon collection of punch models in CD26-ERT2-hDTR mice. b, c Micro-CT images and quantification of ectopic bone in Achilles tendons from diphtheria toxin (DTx) and vehicle control (Veh) group. n = 5 per group. Scale bar: 1 mm. Two-tailed unpaired Student’s t test, t = 11.24, df=8. d–k Histological analysis and quantification of chondrogenic (Sox9 and Col2a1) and osteogenic (Sp7 and Ocn) markers. Scale bar: 100μm. n = 5 biological replicates. Two-tailed unpaired t test, e: t = 6.028, df=7.598; g: t = 4.253, df=8; i: t = 5.114, df=7.998; k: t = 8.008, df=8. l GO pathway analysis of upregulated genes in punched group compared to sham control. Hypergeometric test, FDR correction. m Heatmap of differentially expressed genes from sham and punch group. n GSEA analysis of upregulated genes in punched group compared to sham group. o KEGG pathway analysis of upregulated genes in CD26+ cluster from scRNA-seq data. p Histological analysis and quantification of TNC for Achilles tendon from sham and punched group. n = 5. Scale bar: 100 μm. Two-tailed unpaired Student’s t test, t = 4.987, df=8. q Histological analysis and quantification of p-Yap for Achilles tendon from sham and punched group. n = 5. Scale bar: 100 μm. Two-tailed unpaired Student’s t test, t = 7.044, df=8. r–s Micro-CT analysis and quantification of ectopic bone formation in TNC+/+ and TNC–/– mice 3 months after punched injury. n = 5. Scale bar: 1 mm. Two-tailed unpaired Student’s t test, t = 7.077, df=8. t–u Micro-CT analysis and quantification of ectopic bone formation in Veh. and XMU-MP-1 group 3 months after punched injury. n = 5. Scale bar: 1 mm. Two-tailed unpaired Student’s t test, t = 5.553, df=8. AOD average of density. Data shown are mean ± SEM. Source data are provided as a Source Data file.

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