Fig. 1: Characterization of NAb 7H13 in vivo and in vitro.
From: A single residue switch mediates the broad neutralization of Rotaviruses
a Neutralization profile of 7H13 (murine Fc) and C7H13 (human Fc) against a panel of RV strains. 41# (human Fc) serves as positive control, while 5C6 (human Fc) and A2H4 (murine Fc) are negative controls. Refer to Figure S1 for detailed datasets. Cartoon illustrations were created in BioRender. Yang, H. (2024) https://BioRender.com/c98a036. b Schematic of the experimental design to assess the prophylactic effect of NAbs against RV infection and fecal shedding in a murine model. Created in BioRender. Yang, H. (2024) https://BioRender.com/s21c678. c Protective efficacy of 7H13 and m41# against fecal virus shedding in adult mice (n = 5 per group) following RV EDIM challenge. Data are log10 transformed and presented as mean ± SEM. d Cross-inhibition between C7H13 and m41# as evaluated by blocking ELISA. Blocking mAbs (100 μg/mL) were pre-incubated with microplates before the addition of blocked mAbs, and the binding of blocked mAbs was detected by HRP-conjugated secondary antibodies. Data are shown as mean ± SEM of three technical replicates. e Fold change in neutralizing activity of NAbs over time. Fold change represents the variation in IC50 across time points (1, 2, 4 and 6 h), calculated as the ratio of IC50 at 1 h relative to those at subsequent time points. Refer to Figure S3 for detailed datasets. f Schematic of the experimental setup for pre- and post-attachment neutralization assays. Created in BioRender. Yang, H. (2024) https://BioRender.com/j09b061. g Neutralizing activities of NAbs against SA11 and Wa strains in pre- and post-attachment assays. Data are shown as mean ± SEM of three technical replicates. Source data are provided as a Source Data file.
