Fig. 3: Cryo-ET reveals the disordered VP4 on virion upon 7H13 binding.
From: A single residue switch mediates the broad neutralization of Rotaviruses
a Representative tomographic slice of the native SA11 virion. b Magnified view of VP4 from a, showing intact VP4 morphology. c Segmented SA11 volume with radial coloring to highlight the core-to-periphery gradient. Blue and pink arrows indicate areas detailed in (d and e) respectively. d, e Close-up views of VP4 from the native SA11 virion. The virus is shown as semi-transparent surfaces overlaid with atomic models of RRV strain (PDB: 4V7Q). f Representative tomographic slice of the SA11:7H13 complex after 1-h incubation at 37 °C, showing altered VP4 morphology. g Magnified view of VP4 from (f) showing disrupted VP4 in the presence of 7H13. h Segmented SA11:7H13 volume with radial coloring, highlighting disordered regions of VP4. Blue and pink arrows indicate areas detailed in i and j, respectively. i, j Close-up views of disordered VP4 densities from the SA11:7H13 complex. The complex is shown as semi-transparent surfaces overlaid with composite SA11:7H13 model, generated by superimposing VP4:7H13 structure onto the virus model (PDB: 4V7Q). Scale bars: 50 nm in (a and f); 10 nm in (b and g).
