Fig. 4: Cryo-EM analysis reveals temperature and reacting time are dependent for the destabilization of VP4 by 7H13.
From: A single residue switch mediates the broad neutralization of Rotaviruses
a–f Comparative structural analyzes of the native SA11 virion (a) and SA11:7H13 complexes following incubations under varying conditions: at 4 °C for 20 seconds (b), 5 minutes (c), 1 h (d), 12 h (e), and 4 °C for 1 h followed by 37 °C for another 1 h (f). VP7, VP4, left-7H13, and right-7H13 are colored light gray, pink, green, and blue, respectively. g, h 3D class averages of the SA11:7H13 complex for 20-second (g) and 5-minute (h) incubations, with VP4 represented as semi-transparent surfaces overlaid with ribbon diagrams, color-coded by domain: head in purple, body and stack in pink, and foot in yellow. i Schematic illustration of the hypothesized sequential VP4 disruption process. The color scheme corresponds to that used in h, with VP5* β-barrel domains shown in shades of pink.
