Table 2 VLP yield and thermostability
Expression System | Serotype | VLP Yield (D Ag/100 mL Culture) | Vaccine Doses (per 100 mL Culture) | Thermostability (Temp °C 50% D Ag loss) | Thermostability vs WT Empty Capsids (°C) | Thermostability vs IPV (°C) | D:C Ratio |
|---|---|---|---|---|---|---|---|
Mammalian | PV1-SC6b | 26.3 | 0.8 | 49 | +13 | 0 | D>C |
PV2-SC6b | 76.5 | 9 | 57 | +15 | +5 | - | |
PV3-SC8 | 698 | 25 | 62 | +29 | +10 | D>>C | |
Baculovirus | PV1-SC6b | 398 to 969 | 12–30 | 44 | +8 | −5 | D only |
PV2-SC6b | 127 to 138a | 16–18 | 49.5 | +7.5 | 2.5 | - | |
PV3-SC8 | 935 to 2267 | 33–81 | 60 | +27 | + 8 | D only | |
Yeast | PV1-SC6b | 1030 (±40) | 32 | 43.5 | +7.5 | −5.5 | D>>C |
PV2-SC6b | 69 (±12) | 8 | 52.5 | +10.5 | +0.5 | - | |
PV3-SC8 | 745 (±65) | 26 | 55 | +22 | +3 | D>C | |
Plant* | PV1-SC6b | 235 (±71) | 7 | 44 | +8 | −5 | D>C |
PV2-SC6b | 1.3 (±0.4) | 0.16 | 53 | +11 | +1 | - | |
PV3-SC8 | 180 (±28) | 6 | 54 | +21 | +2 | D>C |
