Fig. 6: Rate limiting protonation steps and radical formation on the NdasCDO-catalysed reaction. | Nature Communications

Fig. 6: Rate limiting protonation steps and radical formation on the NdasCDO-catalysed reaction.

From: Broad substrate scope C-C oxidation in cyclodipeptides catalysed by a flavin-dependent filament

Fig. 6: Rate limiting protonation steps and radical formation on the NdasCDO-catalysed reaction.The alternative text for this image may have been generated using AI.

a Solvent kinetic isotope effects with 91% D2O and with 9% glycerol as a viscosity control yield D2OV/KM-cFG = 1.4 ± 0.2, and D2OV = 2.1 ± 0.1. Data for six replicates are shown. Line is a fit to Eq. (4). b Continuous wave EPR spectra acquired in samples frozen in liquid nitrogen with 5 mM cFG (orange) or cGG (pink) and 100 μM NdasCDO. Controls with cFG only (blue) or NdasCDO only (purple) are also shown. c Coupled reaction with hydrogen peroxidase and ABTS (2,2’-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt), showing hydrogen peroxide formation. d Summarized catalytic cycle depicting a potential neutral flavin semiquinone intermediate.

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