Fig. 8: RNA-FISH analysis of Grem1 and pLMP and dLMP marker genes in pig limb buds. | Nature Communications

Fig. 8: RNA-FISH analysis of Grem1 and pLMP and dLMP marker genes in pig limb buds.

From: Single-cell profiling of penta- and tetradactyl mouse limb buds identifies mesenchymal progenitors controlling digit numbers and identities

Fig. 8: RNA-FISH analysis of Grem1 and pLMP and dLMP marker genes in pig limb buds.

Analysis of the spatial distribution of Grem1, pSMAD, Msx1, and signature genes for pLMPs (Lhx2) and dLMP (Jag1, Tfap2b) pig forelimb buds at D23 (n = 3 independent biological replicates per probe). a Schemes show the pentadactyl digit anlagen in a pig forelimb bud at D30 and the resulting tetradactyl digit pattern at D33 (not drawn to scale, adapted from ref. 44). b–e Pig-specific HCR probes were used for whole-mount RNA-FISH and pSMAD activity was detected by whole-mount immunofluorescence. Bottom panels in (d, e) show the merge of the Lhx2 with the Jag1 or Tfap2b expression domains in the same forelimb bud. All limb buds are oriented with anterior to the top and posterior to the bottom. Scale bars: 300 μm.

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