Fig. 6: Optogenetic manipulation of ovoid cells toggles object preference.
From: Atypical hippocampal excitatory neurons express and govern object memory
A Viral strategy for targeting ovoid (left) and pyramidal cells (right). B Novel object recognition and optogenetic inhibition. C Discrimination indices on test day for inhibition occurring during training session (n = 7 control, n = 7 ovoid, n = 7 pyramidal; ovoid vs. pyramidal p = 0.25, ovoid vs. control p = 0.012, pyramidal vs. control p = 0.06, Kruskal-Wallis test; training inhibition from 0, control p = 0.031, ovoid p = 0.29, pyramidal p = 0.078, two-sided Mann-Whitney U test). Data are presented as mean values ± SEM. D As in (C), but for inhibition during testing (n = 7 control, n = 6 ovoid, 7 pyramidal; ovoid vs. pyramidal p = 0.20, ovoid vs. control p = 8.2e-3, pyramidal vs. control p = 0.20, Kruskal-Wallis test; test inhibition from 0, control p = 0.031, ovoid p = 0.031, pyramidal p = 0.016, two-sided Mann-Whitney U test). E, F As in (A, B), but for ChETA-based ovoid neuron excitation during training, assessing encoding. G, H As in (C, D), but for excitation during testing, assessing short-term retrieval (n = 7 control, n = 7 ovoid, n = 6 pyramidal; Training excitation: ovoid vs. control p = 0.82, ovoid vs. pyramidal p = 0.44, control vs. pyramidal p = 0.72; train excitation from 0, control p = 0.02, ovoid p = 7.8e-3, pyramidal p = 0.022. Test excitation: ovoid vs. control p = 1.5e-2, ovoid vs. pyramidal p = 2.4e-3, control vs. pyramidal p = 0.35, Kruskal-Wallis test; test excitation from 0, control p = 0.031, ovoid p = 7.8e-3, pyramidal p = 0.031, two-sided Mann-Whitney U test). I, J As in (E, F), but for excitation assessing long-term retrieval, occurring 50 days after initial encoding, as well as a subsequent non-excitation retrieval test. K Discrimination indices for preferences during stimulation test day and non-stimulation test day for long-term retrieval (n = 6 control, n = 6 ovoid; p = 8.7e-3, Kruskal-Wallis test; test excitation from 0, test excitation p = 0.031, test non-excitation p = 0.44, two-sided Mann-Whitney U test). Data are presented as mean values ± SEM.
