Fig. 3: Improved metabolite production in Streptomyces sp. with multiplexed genome editing by Cas9-BD. | Nature Communications

Fig. 3: Improved metabolite production in Streptomyces sp. with multiplexed genome editing by Cas9-BD.

From: Engineered CRISPR-Cas9 for Streptomyces sp. genome editing to improve specialized metabolite production

Fig. 3: Improved metabolite production in Streptomyces sp. with multiplexed genome editing by Cas9-BD.The alternative text for this image may have been generated using AI.

a The design of the promoter for the exchange of genes in the oviedomycin BGC in S. antibioticus NRRL 3238. The target genes, ovmOI, ovmOII, and ovmF, are colored red, purple, and green, respectively. The inserted promoters, ermE*p, kasO*p, and sp44p, are also represented. b Results of flask cultivation of the wild-type and engineered S. antibioticus NRRL 3238. Engineered strains with activated oviedomycin BGCs exhibited a dark brown color. c Exchanged promoters of each strain are listed. Blank fields in the table indicate that the promoter was not exchanged. d The design of the promoter for the exchange of genes in rapamycin BGC in S. rapamycinicus NRRL 5491. The genes of polyketide synthase, rapA, rapB, and rapC, are colored green, and the BGC activator gene and rapH are colored red. The inserted promoters, ermE*p, kasO*p, and sp44p, are also presented. e Results of flask cultivation with the wild-type and engineered S. rapamycinicus NRRL 5491 are shown. f Exchanged promoters of each strain are listed. Blank fields in the table indicate that the promoter was not exchanged. All cultivations were performed in triplicates (n = 3). The mean was plotted with error bar representing standard deviation. P values were determined by unpaired two-tailed Student’s t-test (ns, not significant; *, P < 0.05; **, P < 0.01, ***, P < 0.001). Source data are provided as a Source Data file.

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