Fig. 2: Immunohistochemical identification of vagal sensory neurons innervating the liver. | Nature Communications

Fig. 2: Immunohistochemical identification of vagal sensory neurons innervating the liver.

From: Liver-innervating vagal sensory neurons are indispensable for the development of hepatic steatosis and anxiety-like behavior in diet-induced obese mice

Fig. 2: Immunohistochemical identification of vagal sensory neurons innervating the liver.

a AAVrg-FLEX-jGcamp7s viruses were injected into the livers of AvilCreERT2 mice. b,c Images showing co-expression of GFP and Avil in a subset of neurons in the left and right nodose ganglia of AvilCreERT2 mice injected with AAVrg-FLEX-jGcamp7s into the liver. Upper panel: scale bar, 100 μm. Bottom panel: higher magnification view of the yellow square area. Arrowheads represent the vagal sensory neurons that innervate the liver. Scale bar, 10 μm. d,e Schematic illustration of our experimental conditions (d). Images showing PLAP-positive nerve fibers in the liver parenchyma. Right panel: Higher-magnification view of the black square box in the left panel. Scale bars, 200 μm (left), 50 μm (right).

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