Fig. 7: Deleting liver-innervating vagal sensory neurons improves anxiety-like behavior.

a Schematic representation of the experimental configurations. b Graphs showing the time spent in the inner and outer areas and zone alteration in the open filed test between the male groups (n = 9 mice vs. 9 mice, two-tailed t-test, inner, p = 0.0001, outer, p = 0.0001, zone alteration, p = 0.03). c Graphs showing the cumulative duration spent in the open and closed arms in the EPM test between the male groups (n = 5 mice vs. 5 mice, two-tailed t-test, p = 0.004). d Graphs showing the time spent in the light and dark zones and the number of zone transition for control (n = 6 mice) and experimental (n = 6 mice) male mice during the light-dark box assessment. Two-tailed t-test, light, p = 0.01, dark, p = 0.01. e, f Graphs showing that the FST and TST evaluations revealed no significant differences in immobility time between the groups (n = 8 mice vs. 9 mice). g Graphs showing the time spent in the inner and outer areas and zone alteration in the open filed test between the female groups (n = 13 mice vs. 12 mice, two-tailed t-test, inner, p = 0.005, outer, p = 0.5 zone alteration, p = 0.004). h–k Graphs showing no significant differences between females with and without hepatic vagal sensory input across several behavioral assessments. These tests included the EPM test (h, n = 7 mice vs. 6 mice), LD box test (I, n = 7 mice vs. 6 mice), FST (j, n = 6 mice vs. 7 mice), and TST (k, n = 7 mice vs.7 mice). Bar graphs b–k were analyzed using Two-tailed t-tests. Data are presented as mean values ± SEM. Source data are provided as a Source Data file.