Fig. 4: Single cell transcriptomic analysis of unstimulated Sepsecs-specific CD4 T cells. | Nature Communications

Fig. 4: Single cell transcriptomic analysis of unstimulated Sepsecs-specific CD4 T cells.

From: Single cell profiling of circulating autoreactive CD4 T cells from patients with autoimmune liver diseases suggests tissue imprinting

Fig. 4: Single cell transcriptomic analysis of unstimulated Sepsecs-specific CD4 T cells.

A Experimental design for scRNA-seq data analysis of Sepsecs/SLA185-197 HLA-DRB1*0301 pMHCII tetramer positive cells. B Pseudocolor dot plot representation of pMHCII tetramer staining in three of six patients. Top: surface expression of CD45RA and pMHCII tetramer in CD4+ T cells. Bottom: surface expression of PD-1 and pMHCII tetramer in CD4+ CD45RA− T cells (numbers indicate number of CD45RA−Tetramer+ cells per million total CD4 and percentage). C Box plots of gene set score distribution of pMHCII tetramer negative (TTneg, n = 366) or positive (TTpos, n = 75) cells from six AIH patients for antigen reactivity modules as indicated (auto-reactivity, p = 6.4 × 10−14; H1N1, p = 0.011). Each box plot indicates a patient sample. D Box plots of gene set score distribution of pMHCII tetramer negative (TTneg) or positive (TTpos) cells for top marker genes of circulating memory PD-1+CXCR5− CD4 T cell clusters defined in Fig. 3E (Cluster 1, p = 0.51; Cluster 2, p = 1 × 10−9; Cluster 3, p = 0.37; Cluster 5, p = 0.54; Cluster 6, p = 0.64). Data are presented as mean values ± SD. Two-sided, Unpaired Mann-Whitney test was used. Source data are provided as a Source Data file.

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