Fig. 1: Single cell RNA-seq analysis of LMPPs derived from Mir142+/+, Mir142−/−, Mir142+/+BCR-ABL, and Mir142−/−BCR-ABL mice.
From: miR-142 deficit in T cells during blast crisis promotes chronic myeloid leukemia immune escape
a Frequency and number of LMPPs derived from BM of Mir142+/+BCR-ABL mice and Mir142−/−BCR-ABL mice (BCR-ABL were induced by tet-off for 3 weeks; n = 12 mice per group). b–f Schematic design and results. BM LMPP cells were sorted from Mir142+/+, Mir142−/−, Mir142+/+BCR-ABL, and Mir142−/−BCR-ABL mice and subjected to scRNA-seq (b). Fourteen clusters (C) including T-primed, Myeloid-primed, and B/T-primed clusters were identified (c) and expression levels of hematopoietic gene transcription factors and cluster differentiation (CD) antigens in different clusters are shown (d). Distribution of each cluster in LMPPs (e) and expression levels of Notch1, Dntt, and Elane genes in lymphoid-primed C0-3 and myeloid-primed C4 (f) are shown. B/A BCR-ABL, LMPP lymphoid-primed multipotent progenitors, tet tetracycline. For a comparison between groups was performed by two-tailed, unpaired t-test. Results shown represent mean ± SEM. Source data are provided as a Source Data file.
