Fig. 6: M-miR-142 restores T cell antileukemic activity.
From: miR-142 deficit in T cells during blast crisis promotes chronic myeloid leukemia immune escape
a Representative plots and combined results of uptake of Cy3-conjugated M-miR-142 by human and mouse T cells (in vitro: 1μΜ; in vivo: 30 mg/kg; n = 3). b–e. Experimental design and results. NSG mice were co-transplanted with Mir142−/−BCR-ABL LSK+Mir142−/− T cells and then treated with SCR or M-miR-142 for 3 weeks (b), circulating T and leukemic cell counts (c), circulating blasts (%) and blood smear (d), and survival (e) were shown (n = 10 per group). f–h. Experimental design and results. Mir142−/−BCR-ABL mice were treated with SCR or M-miR-142 for 3 weeks (f), then BM T cells (n = 7 per group), WBC counts (n = 8), PB LSKs (n = 5), and survival (n = 14 for SCR group; n = 13 for M-miR-142 group) were shown (g). Leukemic cell engraftment (n = 9) and survival (n = 10) of the 2nd recipients were shown (h). i–l Experimental design and results. Two cohorts of BC CML PDX were given autologous human T cells and then treated with SCR or M-miR-142 (30 mg/kg/day, iv) for 3 weeks (i). Human T and leukemic cell engraftment in PDX-1 (j; n = 8 for SCR and T+ SCR groups; n = 12 for M-miR-142 and T+M-miR-142 groups) and PDX-2 (k; n = 8 for SCR and M-miR-142 groups; n = 14 for T+ SCR and T+M-miR-142 groups) and the survival of PDX-2 (k; n = 7 for SCR and M-miR-142 groups; n = 9 for T+ SCR and T+M-miR-142 groups) were shown. PB human leukemic cell engraftment and survival (l; n = 8 for SCR and M-miR-142 groups; n = 9 for T+ SCR and T+M-miR-142 groups) of the 2nd recipients receiving BM cells from the treated PDX-2 were shown. SCR scramble RNA, LSK Lin−Sca-1+c-Kit+, PDX patient-derived xenograft. For c, d, g, h, j and k, comparison between two groups was performed by two-tailed, unpaired t-test. For j–l, comparisons among multi-groups were performed by one-way ANOVA. For e, g, h, k and l, log-rank test was used to compare two or more survival curves. P values were corrected for multiple comparisons using Holm–Šídák method. Results shown represent mean ± SEM. For b, f and i, mouse images were created in BioRender. Chen, F. (2025) https://BioRender.com/e61c469. Source data are provided as a Source Data file.
