Fig. 2: Departure from randomness through self-assembly of aminoacyl phosphate esters.
From: Selective peptide bond formation via side chain reactivity and self-assembly of abiotic phosphates
A Peptide bond formation from 2a or 2b with amino acid mixtures highlighting the reactivity trend of 2a, 2b, 2c, and 2d. B Cryo-transmission electron microscopy images (from left to right) of 10 mM 2b immediately after dissolving, after 40 min, after 15 days, and upon mixing 2b with R. Peptide bond formation between (C) 2a and (D) 2b with amino acid mixture I. The concentration used for 2a or 2b was 10 mM, while the total concentration for the amino acids in the mixture was 50 mM (10 mM each). Striped bars indicate hydrolysis products (2a-OH or 2b-OH). Peptide coupling yields were measured at the end of each reaction: for 2a, the reaction completes in 30 min, whereas 2b required 48 h. E Time-dependent hydrolysis of aminoacyl phosphate esters. Error bars represent the standard deviation of three independent experiments.
