Fig. 2: Generation and optimization of blastoid formation from higher passage ESC lines.
From: Highly efficient construction of monkey blastoid capsules from aged somatic cells
a Representative phase-contrast images of ESCs aggregates at indicated time points during blastoid formation. Scale bars, 200 μm. b and c Representative phase-contrast images of monkey blastoids (b) and monkey blastocyst-E9 (c). Scale bars, 200  m. d Blastoid formation efficiency from M-ESCs, mean ± S.E.M. of 5 biological replicates. e The effect of HDM components, indicated as a percentage of HDM concentration, on blastoid formation efficiency during the TSG stage. Scale bars, 200 μm. f–i Representative phase-contrast images of higher passage ESCs aggregates from different starting cell numbers (100,000, 1E5; 200,000, 2E5) and HDM culture time at indicated time points during blastoid formation. Scale bars, 200 μm. j Blastoid formation efficiency from higher passage M-ESCs at different M-ESC starting cell numbers and HDM culture time, n = 6 technical replicates. Error bars, mean ± S.E.M. Two-tail t - test. k Diameter of blastoids at higher passage M-ESCs number aggregates and HDM culture time during blastoid formation, n = 47 technical replicates. Error bars, mean ± S.E.M. Two-tail t - test. l Representative immunofluorescence co-staining images of GATA3, OCT4 and GATA6 (3 independent replicates). Scale bars, 100 μm. m Representative immunofluorescence images of ZO-1 in monkey blastoids (3 independent replicates). Scale bars, 100 μm. n Representative immunofluorescent staining images of GATA3/SOX2/SOX17 in monkey blastoid (top) and in monkey blastocyst-E9 (bottom). Scale bars, 100 μm. o Representative immunofluorescent staining image of GATA2/GATA3/NANOG in monkey blastoid (top) and blastocyst (bottom). Scale bars, 100 μm. p Representative immunofluorescent staining image of OCT4 in monkey blastoid (top) and blastocyst (bottom). Scale bars, 100 μm. q Representative immunofluorescent staining image of CCR7 in monkey blastoid (top) and blastocyst (bottom). Scale bars, 100 μm. r Representative immunofluorescent staining image of CDX2 in monkey blastoid (top) and blastocyst (bottom). Scale bars, 100 μm. 3 independent replicates for (n–r).
