Fig. 5: Generation of monkey blastoids from M-nt-ESCs and M-iPSCs.
From: Highly efficient construction of monkey blastoid capsules from aged somatic cells
a–d Representative phase-contrast images of old and young nt-ESCs and iPSCs aggregates at indicated time points during blastoid formation. Scale bars, 200 μm. e–h Representative immunofluorescence images of GATA3, OCT4, and GATA4 in monkey blastoids from old and young nt-ESCs, old and young iPSCs. Scale bars, 100 μm. i–l Representative immunofluorescence images of ZO-1 in monkey blastoids from old and young nt-ESCs, old and young iPSCs. Scale bars, 100 μm. m Representative phase-contrast image of nt-ESC blastoids on day 5 (left) and blastoid formation efficiency (right) of M-nt-ESCs (n = 3 biological replicates for old and n = 3 biological replicates for young). Scale bars, 200 μm. Error bars, mean ± S.E.M, unpaired two-tailed t test. n Representative phase-contrast image of blastoids on day 5 (left) and measurement of the x/y ratio of blastoids from nt-ESCs (n = 30 technical replicates for old and n = 30 technical replicates for young). Scale bars, 200 μm. Error bars, mean ± S.E.M, unpaired two-tailed t test. o Representative phase-contrast image of blastoids on day 5 (left) and blastoid formation efficiency of monkey iPSCs (n = 2 biological replicates and 3 technical replicates for old and n = 2 biological replicates and 3 technical replicates for young). Scale bars, 200 μm. Error bars, mean ± S.E.M, unpaired two-tailed t test. p Representative phase-contrast image of blastoids on day 5 (left) and measurement of the x/y ratio of blastoids from monkey iPSCs (n = 30 technical replicates for old and n = 30 technical replicates for young). Scale bars, 200 μm. Error bars, mean ± S.E.M, unpaired two-tailed t test.
