Fig. 5: SAXS of phase-separated Bik1.
From: Phase separation of a microtubule plus-end tracking protein into a fluid fractal network
A SAXS measurement (blue curve) with Gaussian-blob fit (red curve) and fit obtained with 50,000 model 3 copies (black curve). The model 3 fit was used to model the fractal structure and is restricted to q > 2π/σw due to the Gaussian window with σw = 1500 Å. The straight blue line above the SAXS measurement represents the I(q)~q−2 behavior expected for a fractal network with a fractal dimension of 2. The green curve shows the Bik1 form factor obtained from our SEC-SAXS data (Fig. 2A). Inset, schematic representation of model 3. B Radial distribution function of the centers of 50,000 model 3 copies. The decay for r larger than the nearest-neighbor peak is expected for a fractal network. Inset, two model 3 copies with their centers of mass (dots). The red double arrow highlights the distance r between their centers. C Sections taken from the fractal structure obtained with 50,000 model 3 copies. Source data for all panels are provided in the Source Data file.
