Fig. 10: Transcriptome analysis of PontincTG mice following Angiotensin II infusion indicated that Pontin might regulate Hippo pathway in the transgenic mouse model.
A Volcano plot showing the number of differentially expressed genes (DEGs) in heart tissues of PontincTG mice compared to WT littermates following stimulation with Ang-II (1.5 mg/kg BW/day for 2 weeks (n = 3 mice in each group)). B Ingenuity pathways analysis (IPA) DEGs showed enrichment of sets of genes related to specific cellular functions. The negative log of P-values are shown in X-axis. The size of the dots represents the number of DEGs in each category. C GSEA analysis indicated that the top-ranked gene set that was differently expressed in PontincTG mice was “Regulation of Hippo Signalling” based on net enrichment score (NES) and FDR values. D Enrichment plot from GSEA analysis showing the NES and FDR values of gene set “Regulation of Hippo Signalling”. E IPA analysis identified TEAD1 as the top transcription factor that was differently regulated in PontincTG hearts following Ang-II stimulation. Analysis of individual genes revealed that the expressions of (F) MOB1 and (G) LATS1 were significantly downregulated in PontincTG mice, whereas expression of (H) MST1 showed trend of reduction although it did not reach significance. (n = 3 mice in each group). Data are presented as mean ± SEM. Statistical tests used: (F–H) two-tailed Student’s t-test. Source data are provided as a Source Data file.
