Fig. 5: Transcriptomic and pathway screening analysis identifies Hippo pathway to be differentially regulated following Pontin knockout.

A Volcano plot of differentially expressed genes (DEGs) in Pontin^icKO hearts compared to controls at 1 week and (B) 3 weeks following tamoxifen injection (n = 3 mice in each group). C Ingenuity pathway analysis (IPA) of cellular functions of DEGs at 1 week after Pontin knockout and (D) at 3 weeks after Pontin knockout. The enriched cellular functions are written in Y-axis. The X-axis represents negative log of P-values. The size of the dots represents the number of DEGs in each category. E Results of pathway screening assays in H9c2 cardiac myoblast cell lines using Pontin overexpression and (F) Pontin silencing systems demonstrated a consistent modulation of YAP activity by Pontin (n = 5 independent experiments in each group). G Gene set enrichment analysis (GSEA) - enrichment plot of gene set ‘Hippo signalling pathway’ at 1 week after Pontin knock out and (H) at 3 weeks after knockout indicating significant enrichment of this gene set following Pontin knockout. Data are presented as mean ± SEM. Statistical significance was determined by multiple two-tailed Student’s t-test (E&F). Source data are provided as a Source Data file.