Fig. 6: Expression analysis of cardiac transcripts and proteins from WT and PontinicKO mice revealed differential expression of members of Hippo pathway.
A Selection and classification of Hippo pathway components based on signalling pathway database (KEGG database). Genes are classified as Hippo pathway core components, negative regulator of Hippo pathway, positive regulator of Hippo pathway, Hippo pathway effector (YAP/TAZ) and inhibitor of YAP/TAZ. B Heat map showing cardiac expression of Hippo pathway components in controls vs PointinicKO mice at 1 week and 3 weeks after Pontin deletion (n = 3 mice in each group). The data indicated an increased in the expression of core Hippo components in PontinicKO hearts partularly at 3 weeks after tamoxifen injection. Furthermore, individual transcript analysis of key components of Hippo pathway revealed that the expression of (C) MST1, (D) SAV1 and (E) MOB1 were significantly upregulated at 3 weeks after Pontin deletion in cardiomyocytes (n = 3 mice in each group). F Representative Western blots to assess protein expression of key components of Hippo pathway in PontinicKO (αMCM-Ponflox + tamoxifen) vs controls (the other 3 groups). Analysis of band density of (G) MST1, (H) LATS1, (I) YAP and (J) MOB1 indicated a significant increase in the expression of MST1 and MOB1 in PontinicKO mice (n = 5 mice in each group). K Representative images of immunohistochemistry analysis to detect phospho-YAP and total YAP in heart tissue sections of PontinicKO (MCM-Ponfloxed + TAM) and control mice (MCM-Ponfloxed without tamoxifen)(scale bars = 20μm). L Quantification of signal intensity indicated a trend of increased phospho-YAP activity in PontinicKO mice (n = 4 mice in each group). Data are presented as mean ± SEM. Statistical tests used: (C–L) one-way ANOVA followed by multiple comparisons test (Tukey’s), (L) Student’s t-test. Source data are provided as a Source Data file.
