Fig. 6: Annotation results and downstream analyses on the MISAR-seq MB dataset.

a The provided annotations of the S1 slice of developmental stage E18.5 and the annotation outcome of the corresponding S2 slice based on the integration results of INSTINCT. b GO analysis performed for the two rare spot-types, subpallium_2 and muscle, identified in the S2 slice. Adjusted P-values are calculated using the GSEApy package, with enrichment scores evaluated using a two-sided test. c Results of expression enrichment analysis on the spot-type-specific peaks of diencephalon_and_hindbrain and background peaks. The top 30 significantly enriched tissues are displayed. The vertical dashed lines indicate the threshold for one-sided P-values at the 0.05 significance level, while the solid lines represent the cutoffs for one-sided P-values at the 0.05 level, adjusted using the Bonferroni correction. The heatmaps display Pearson correlation coefficients for pairs of expression profiles, which are organized based on hierarchical clustering using the unweighted pair-group method with arithmetic means. d The results of partitioned heritability analysis performed on the spot-type-specific peaks and background peaks for three diseases, including ADHD, schizophrenia, and autism. The error bars represent the jackknife standard errors, calculated over 200 equally sized blocks of adjacent SNPs, and the centers of the bars indicate the average enrichment estimates. The enrichment score and standard error for a domain are set to 0 if the enrichment score is less than 0. Source data are provided as a Source Data file.