Fig. 9: Dextran sulfate sodium (DSS)-induced colitis in mice.
a Experimental strategy. 7–8-month-old Tert+/+ (wildtype C57BL/6 J) and Terth/h (G4.8 h) mice were given drinking water with or without 3% DSS for 6 days, followed by 1 day of pure drinking water. Intraperitoneal EdU injection was performed 2 h before tissue collection. b Representative images of colons and spleens following DSS treatment. c Spleen weight. Spleen weight was normalized to the body weight of each mouse. Each data point represents one animal. n = 4. d EdU staining of colon crypt sections. Colon tissues were labeled with anti-EdU (white) and E-cadherin (green) antibodies, as well as Hoechst dye for nuclear staining (blue). Scale bar represents 100 µm. Representative images are shown. e Quantification of EdU-positive cells. Each datapoint in panel e represents the average number of EdU-positive cells per colon crypt in 30 crypts from one animal. n = 6. Means ± SDs are shown in panels c, e. P values, calculated using two-way Anova, are shown. Source data for panels c and e are provided as Source Data files.
