Fig. 2: Repli-ID identifies known and new regulators of DNA replication fork progression/stability.

a Outcome of Repli-ID for Pol ε-9xMyc in 2905 yeast mutants (Supplementary Data 1). Scatter plot shows IP/input ratios at 40 and 80 min after G1 arrest and release in 200 mM HU. The mean of n = 2 independent Repli-ID screens is shown. Each dot represents a single mutant strain. Increased (light blue) and decreased factors (light orange), known replication factors (black) and validated factors (red) are highlighted. b GO Slim biological process analysis (false discovery rate (FDR) < 0.05) of the top 423 mutants showing depletion of Pol ε (< −1.25 log2(fold change) for t = 40 min and t = 80 min). Genome frequency is depicted in red and frequency within Repli-ID in blue. c ChIP-qPCR analysis of Pol ε-9xMyc at ARS607 and ARS404 in a selection of yeast mutants from the Repli-ID screens at 40 min after G1 arrest and release in S-phase in 200 mM HU. Data represent the mean relative fold enrichment of Myc signal over beads only signal of n = 2 independent experiments. Values were normalized to a non-replicated region (ARS607 + 14 kb). d ChIP-qPCR analysis of Pol ε-9xMyc in selected hits from the Repli-ID screens, for which the corresponding mutants were generated de novo in the W303 strain background, at the indicated origins 20 min after G1 arrest and release in S-phase in 200 mM HU. Data represent the mean relative fold enrichment + SEM of Myc signal over beads only signal in n = 3 or n = 4 independent experiments. Values were normalized to a non-replicated region (ARS607 + 14 kb). Statistical significance compared to the wild type was calculated using the two-tailed unpaired Student’s t test, assuming unequal variances, *p < 0.05, **p < 0.01. Source data are provided as a Source Data file.