Fig. 5: Antibody binding and inflammation markers in an ABOi KT model. | Nature Communications

Fig. 5: Antibody binding and inflammation markers in an ABOi KT model.

From: Enzymatic conversion of blood group B kidney prevents hyperacute antibody-mediated injuries in ABO-incompatible transplantation

Fig. 5: Antibody binding and inflammation markers in an ABOi KT model.The alternative text for this image may have been generated using AI.

a Immunofluorescence images showing antibody deposition in glomeruli before and after reperfusion. Scale bars, 20 μm. Following 4 h of reperfusion, substantial amounts of IgM and IgG were deposited in the control kidneys (without B-zyme treatment), whereas only minimal IgM deposition was observed in the ECO group (10 μg/mL B-zyme treatment). b Relative MFI of IgM and IgG deposition in control vs. ECO kidneys. Six fields of view were selected per kidney (n = 3) for 18 images total per sample. c Histograms depicting anti-B IgM and IgG levels in the perfusate collected before and after 4 h of reperfusion in the control and ECO groups. d Changes in MFI values at all time points following reperfusion based on reperfusion 5 min (n = 3). e Changes in inflammation markers in the perfusate after 4 h of reperfusion in control vs. ECO groups (n = 3). All data are presented as mean ± SEM. Data in b and d were analyzed by two-way ANOVA and Bonferroni’s multiple comparisons test. Data in e were analyzed using two-tailed paired t test. ns, no significance. Source data are provided as a Source Data file. ABOi ABO incompatible, KT kidney transplantation, ECO enzyme converted O, h hour, MFI mean fluorescence intensity.

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