Fig. 8: Histopathological and immunological assessments of biopsy samples at various timepoints post-transplantation.

a Biopsy from ECO allograft protocol. b H&E staining of ECO kidney pre-transplantation. Scale bars, 50 μm. c H&E staining of ECO allograft at 63 h post-transplantation. Scale bars, 50 μm. Black arrows show interstitial leukocytes infiltration. d Quantification of kidney injury scores before and after 63 h post-transplantation. Scores were derived from six randomly selected visual fields per sample (n = 1). e Immunofluorescence staining showing expression of B-Ag and H-Ag in ECO glomeruli before and after transplantation. Scale bars, 20 μm. B-Ag re-expression observed at 48 h post-transplantation. f Relative changes in MFI of B-Ag and H-Ag compared to pre-transplantation levels. Six fields of view were selected per kidney (n = 1) for 6 images total per timepoint. g Immunofluorescence staining of immunological markers in ECO allograft before and after transplantation. Scale bars, 20 μm. IgM, IgG, C1q, and C3 deposition observed in ECO graft at 63 h post-transplantation, with no detectable activation of C4d and C5b-9. h MFI values normalized to pre-transplantation levels at 63 h post-transplantation. Six fields of view were selected per kidney (n = 1) for 6 images total per group. All data are presented as mean ± SEM. Data in d and h were analyzed using two-tailed paired t test. Data in f were analyzed using one-way analysis of variance (ANOVA) and Bonferroni’s multiple comparisons test. ns, no significance. Source data are provided as a Source Data file. ECO enzyme converted O, H&E hematoxylin and eosin, h hour, B-Ag B antigen, H-Ag H antigen, MFI mean fluorescence intensity.