Fig. 1: Characterization of LXRα W441F in vitro.

Immortalized bone marrow derived macrophages were infected with adenovirus expressing LXRα or LXRα W441F. After infection, cells were treated for 24 h with 10 µM 24(s),25-epoxycholesterol or 1.0 µM T0901317, RNA was isolated and the mRNA levels of a Srebp1c and b Scd1 were measured by real-time PCR as described in the Methods section. *Statistically significant difference between vehicle and treated group for each virus determined by two-way ANOVA (p ≤ 0.05, n = 4). Each point represents an independent experiment with mRNAs measured in triplicate. Boxes extend from the 25th to 75th percentiles. The center line in the middle of the boxes represents the median. Whiskers extend from the lowest to the highest point. c Cells were infected as described above and treated with different concentrations of T0901317 for 24 h. RNA was isolated and Srebp1c mRNA levels were measured by real-time PCR as described in the Methods section. Data is the average of 3 independent experiments with Srebp1c mRNA measured in duplicate. Error bars represent the standard deviation. *Statistically significant difference between LXRα and W441F determined by two-way ANOVA (p ≤ 0.05, n = 3). d Nuclear extracts were prepared from Immortalized bone marrow derived macrophages 24 h after infection. LXRα and TBP levels were examined by Western blotting. e LXRα protein levels were quantified from Western by normalization to TBP using ImageQuant TL. Each point is an individual infection. The amount of wildtype LXRα was set at 1. *Statistically significant difference between LXRα and W441F determined by two-tailed Mann–Whitney test (p ≤ 0.05, n = 4). Boxes extend from the 25th to 75th percentiles. The center line in the middle of the boxes represents the median. Whiskers extend from the lowest to the highest point. Source data are provided as Source Data file.