Fig. 8: Comparison of LXRα W441F and LXRα knockout mice.

Male 11–13 week old control (W/W), heterozygous (W/F), homozygous (F/F) W441F mice and Lxrα^–/– mice (AKO) were fed the MASH diet for 4 weeks. a–d Liver gene expression measured by real-time PCR. *Statistically significant difference between control (W/W) and mutant mice; **statistically significant difference between AKO and W441F mutant mice determined by one-way ANOVA (p ≤ 0.05, W/W = 5, W/F = 7, F/F = 8, AKO = 5). e Representative Oil Red O stained liver sections. f, g Liver cholesterol and triglycerides. *Statistically significant difference between control (W/W) and mutant mice; **statistically significant difference between AKO and W441F mutant mice determined by one-way ANOVA (p ≤ 0.05, W/W = 5, W/F = 8, F/F = 5, AKO = 6). h–j Liver gene expression measured by real-time PCR. *Statistically significant difference between control (W/W) and mutant mice; **statistically significant difference between AKO and W441F mutant mice determined by one-way ANOVA (p ≤ 0.05, W/W = 5, W/F = 7, F/F = 8, AKO = 4). k Plasma AST. *Statistically significant difference between control (W/W) and mutant mice; **statistically significant difference between AKO and W441F mutant mice determined by one-way ANOVA (p ≤ 0.05, W/W = 5, W/F = 7, F/F = 8, AKO = 5). l Representative Picrosirius Red stained liver sections. m Quantification of the % surface area positive for Picrosirius Red. *Statistically significant difference between control (W/W) and mutant mice; **statistically significant difference between AKO and W441F mutant mice determined by one-way ANOVA (p ≤ 0.05, n = 5/group). For all whisker plots in the figure, boxes extend from the 25th–75th percentiles. The center line in the middle of the boxes represents the median. Whiskers extend from the lowest to the highest point. Source data are provided as Source Data file.