Fig. 9: Hepatocyte-selective expression of LXRα W441F promotes MASH.
Female 9 week old Rosa26-LSL-FLAG-LXRα W441F/Lxrαfl/fl mice were infected with AAV-TBG-GFP or AAV-TBG-Cre viruses. Mice were maintained on a normal chow diet for 2 weeks and then fed the MASH diet for 4 weeks. a Experimental schematic. b Liver nuclear extracts were prepared as described in the Methods. FLAG-LXRα and TBP levels were examined by Western blotting. c, d Representative H&E stained liver sections. e, f Liver triglycerides and cholesterol were measured as described in the methods section. *Statistically significant difference between GFP and Cre mice determined by unpaired two-tailed t-test (p ≤ 0.05, n = 6/group). g, h Representative Oil Red O stained liver sections. i, j Representative Picrosirius Red stained liver sections. k Quantification of the % surface area positive for Picrosirius Red in liver sections as described in the Method section. *Statistically significant difference between GFP and Cre mice determined by unpaired two-tailed t-test (p ≤ 0.05, n = 6/group). l Plasma ALT measured as described in the Methods section. *Statistically significant difference between GFP and Cre mice determined by two-tailed Mann–Whitney tests (p ≤ 0.05, n = 6/group). m–t Liver gene expression measured by real-time PCR as described in the Methods section. *Statistically significant difference between GFP and Cre mice determined by unpaired two-tailed t-test or two-tailed Mann–Whitney test (p ≤ 0.05, n = 6/group). For all whisker plots in the figure, boxes extend from the 25th–75th percentiles. The center line in the middle of the boxes represents the median. Whiskers extend from the lowest to the highest point. Source data are provided as Source Data file.
