Fig. 5: Gene structure and expression of SIRPB1-SIRPD. | Nature Communications

Fig. 5: Gene structure and expression of SIRPB1-SIRPD.

From: Retrotrans-genomics identifies aberrant THE1B endogenous retrovirus fusion transcripts in the pathogenesis of sarcoidosis

Fig. 5: Gene structure and expression of SIRPB1-SIRPD.

a Long-read RNA-seq of SIRPB1-SIRPD (above). Genomic structure of SIRPB1 and SIRPD (below). b Amino acid sequence of SIRPB1-SIRPD. Magenta literature denotes the part identical to SIRPB1 amino acid sequence (O00241-1, amino acids 1-144) and cyan literature denotes the part identical to SIRPD amino acid sequence (Q5TFQ5, amino acids 25-198). c Predicted domains of SIRPB1, SIRPB1-SIRPD and SIRPD using SMART66 (https://smart.embl.de/). The C-terminal region colored with dark blue in SIRPB1 indicates the transmembrane domain. d Alphafold2 prediction of 3D structure of SIRPB1-SIRPD. e UMAP projection of scRNA-seq data from individuals with cutaneous sarcoidosis. Relative expression of SIRPB1-SIRPD. f RT-qPCR assay. We compared the relative expression of target genes between SM (n = 14) and non-SM (n = 29), followed by two-sided permuted Brunner-Munzel test and Benjamini-Hochberg correction. The experiment was performed at least two times, and the similar results were confirmed. Each dot denotes the relative expression level of SIRPB1-SIRPD to TBP mRNA of individuals. Error bar means the standard deviation value. Source data are provided as a Source Data file. g Measurement of discrimination ability of SIRPB1-SIRPD by ROC-AUC curve.

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