Fig. 6: Mitochondrial retention and erythroid maturation suggest ineffective erythropoiesis in HbGS compared to HbAS. | Nature Communications

Fig. 6: Mitochondrial retention and erythroid maturation suggest ineffective erythropoiesis in HbGS compared to HbAS.

From: Base editing HbS to HbG-Makassar improves hemoglobin function supporting its use in sickle cell disease

Fig. 6

The box plots (panels a, c–e) show median (middle line), 25th percentile (upper line), 75th percentile (lower line), and whiskers to include all observations. a Mitochondrial retention (mean fluorescence intensity, MFI) measured using MitoTracker Deep Red+ flow panel (**p = 0.002). The data was analyzed with the two-sided median test and p-values were adjusted for multiple comparisons. b MitoTracker and TER119+ representative flow histogram. c–e Erythroid cell maturation in the bone marrow was measured as % TER119+ cells in each population (Pro – RBC, I -VI) divided by comparisons of interest (c) HbAA vs HbGG, (d) HbAS vs HbGS (*p = 0.014, **p = 0.004), (e) HbGS vs HbSS. Each maturation stage of the comparison group was compared using the two-sided Mann-Whitney test and p values adjusted for multiple comparisons. For sample size information please see Supplementary Table 5. Source data are provided as a Source Data file.

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