Fig. 7: Batch effects simulation and evaluation. | Nature Communications

Fig. 7: Batch effects simulation and evaluation.

From: Automated cytometric gating with human-level performance using bivariate segmentation

Fig. 7: Batch effects simulation and evaluation.The alternative text for this image may have been generated using AI.

A Distribution shifts on selected protein channels, indicating the simulated batch effects caused significant shifts in protein expression. B UMAP visualization of protein expression from original and noisy data combined. C Same UMAP visualization showing cell-type annotation defined by consensus gating. D F1 evaluation of 9 gates from mass cytometry for all comparison methods, with an overall F1 score comparison at the end. Source data for (A, D) are provided as a Source Data file.

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