Fig. 10: Treatment with MDC-735 therapy does not affect organ anatomy or normal cells.

a Histopathological examination of different types of tissue stained with Hematoxylin and Eosin (H&E) in mice with breast metastasis to the lungs (EMT6) treated with allogeneic mouse MDC-735: vehicle control group (PBS control) and treatment groups (MDC-735 loaded with HFt-735 at 0.25 mg/ml and 0.50 mg/ml). Representative images of n = 4 samples in PBS and n = 3 samples per MDC-735 group. Scale bar = 20 µm. b Histopathological examination of different types of tissue stained with Hematoxylin and Eosin (H&E) in mice dosed subcutaneously with MDC-735 (n = 3). 28 days later animals were euthanized, and liver, lung, spleen, and skin tissue resected and processed to FFPE, 4 µM sections were sectioned and H&E stained. Scale bar = 300 µm (8x), 350 µm (10x) or 250 µm (20x). c and (d) Comparison of normal cells vs. cancer cell line viability in co-culture with MDC-735. Bar plots show the viability of various types of normal cells and the EMT6 cancer cell (c) and the LN-229 cancer cell line (d) after 48-h co-culture with MDC-735. Viability was measured as the number of live cells relative to the control condition (no macrophages). Data represents the mean ± SEM from n = 3 independent replicates. Statistical analysis was performed using one-way ANOVA and post hoc Tukey HSD test; *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.001. Source data are provided as a Source Data file.