Fig. 1: An overview of feature categories and targets.
From: Polarity-JaM: an image analysis toolbox for cell polarity, junction and morphology quantification
A Endothelial cells under static conditions exhibit a random polarity, while endothelial cells exposed to laminar flow polarise against the direction of flow (indicated here by the relative nuclei-Golgi polarity) and elongate. The flow direction is indicated by the black arrow. B Categories of image-based features comprise identification and localisation, polarity, morphology, and intensity readouts, which can be applied to (C) several targets, including cell, nucleus, organelle, marker and junction interfaces. For example, localisation can be computed for the targets cell, nucleus, and organelle. Similarly, morphology features describing the elongation of an object can be calculated for different targets, such as the cell and nucleus. Polarity-JaM features are generated automatically based on available targets and user configuration. See Supplementary Tables 1 and 2 for an overview.
