Fig. 3: Cell and nuclei shape orientation.
From: Polarity-JaM: an image analysis toolbox for cell polarity, junction and morphology quantification
A Example input image with DAPI (yellow) and VE-cadherin (blue). B Cell elongation is indicated by colour with blue ‘roundish’ and red ‘very elongated’. C Cell shape orientation is visualised by a circular colour scheme ranging from 0° to 180°. D Comparison of different flow conditions static, 6 dyne/cm2 and 20 dyne/cm2. E The orientation of cell shape is summarised in circular histograms with statistical read-outs including the polarity index (red bar) and V-score (black bar), lower hemirose plots are duplicated data points and therefore shown transparent. F Histogram showing the change of cell elongation with respect to the three different flow conditions. G Pictogram showing the calculation of the length-to-width ratio and shape orientation, which is applied to each single cell mask (target). Note, that the number of biological replicates is n = 2 for static, n = 1 for 6 dyne/cm2 and n = 1 for 20 dyne/cm2. The full set of features with target cell can be found in Supplementary Table 6. Source data are provided as a Source Data file.
