Fig. 4: Flow-induced NOTCH1 signaling asymmetry.
From: Polarity-JaM: an image analysis toolbox for cell polarity, junction and morphology quantification
A Left shows the NOTCH1 signal (orange) combined with the junction channel (blue). Middle shows the NOTCH1 (here called marker) cue directional intensity ratio. The blue circle inside each cell marks the centroid of the cell, while the vertical dashed red lines are perpendicular to the flow direction and thus separate the cell to front and back. Right shows the marker polarity as a directional property. B Shows the cue directional intensity ratio of the NOTCH1 signal with respect to the flow direction before [left], after 30 min [middle], and after 2 h [right] of exposure to a shear stress level of 20 dyne/cm2. C The circular histogram of the polarity of the NOTCH1 signal before applying a shear stress level of 20 dyne/cm2 [left], after 30 min of exposure [middle], and after 2 h [right]. The red arrow points towards the collective mean direction of polarity, and its length represents the polarity index. Dashed lines indicate the 95% confidence intervals of the circular mean. Black dots indicate single-cell measurements. D Correlation between marker polarity and directional intensity ratio. Note that for the correlation plot, all three conditions are pooled, while the number of biological replicates for each of the flow conditions is n = 1. E Schematic representation of the feature and target for this figure. For an overview of features that can be extracted from intensity measures, see Supplementary Table 7. Source data are provided as a Source Data file.
