Fig. 5: Localised single-cell fluorescence intensity quantification.
From: Polarity-JaM: an image analysis toolbox for cell polarity, junction and morphology quantification
A Quantification of the intensity of KLF4 antibody staining. Overlay of KLF4 (Orange) and VE-Cad (blue) channel (Contrast enhanced for optimal view)[left], KLF4 intensity in the nuclei [middle] and in the cytosol without nucleus [right]. B Intensity ratio of the KLF4 reporter in the nucleus and in the cytosol was colour coded for each single cell. C Comparison of the change in intensity ratios using the DABEST method shows a difference between static conditions and endothelial cells exposed to flow after 4 h and 16 h. The total number of cells analyzed is Ncell = 2860, Ncell = 3467, and Ncell = 3172 for static, 4 h, and 16 h, respectively. Each point indicates an image of n = 2 biological replicates for each condition. Black bars indicate 95% confidence intervals of the mean difference. D Schematic representation of the features and target for this figure. For an overview of features that can be extracted from intensity measures, see Supplementary Table 7. Source data are provided as a Source Data file.
