Fig. 6: Junction morphology quantification.
From: Polarity-JaM: an image analysis toolbox for cell polarity, junction and morphology quantification
A Examples of endothelial cells in static condition, exposed to 6 dyne/cm2 and 20 dyne/cm2. VE-cadherin staining is shown in blue, DAPI in yellow. B Pictograms of quantities extracted from the images. C Statistical comparison of three normalised morphological junction features interface occupancy [left], intensity per interface area [middle] and cluster density [right] demonstrate a significant change in junction dynamics after exposure to flow. Black bars indicate 95% confidence intervals of the mean difference. An overview of features that can be extracted from cell-cell junctions is shown in Supplementary Table 8. Source data are provided as a Source Data file.
