Fig. 1: Secreted Candida albicans Als3 protein induces immune responses.

a Survival curves of male mice infected with 7.5 × 10⁴ cells of WT (SN250), the als3^∆/∆ mutant, or the als3^GPI∆/∆ mutant. P values were calculated using a log-rank (Mantel-Cox) test (n = 16). The data represent the combined results of two independent experiments. ns, not significant. b–e ELISA analysis of secreted cytokines of BMDCs after infections with yeast cells at MOI 1 for 24 h (b–d) or stimulation with purified Als3 (15 µg ml⁻¹), Als3^S170Y (15 µg ml⁻¹) and N-Als3 (5.3 µg ml⁻¹) for 24 h (e). The data in (b–e) represent the mean ± s.e.m. from three independent experiments with two or three technical repeats (b, n = 8; d, e, n = 9). The results in (c) were from two independent experiments with two or three technical repeats (SN250 and als3^∆/∆, n = 5; als1^∆/∆ and als5^∆/∆, n = 6). P-values were calculated using a one-way ANOVA, followed by Tukey’s post-hoc test (b–d) or an unpaired two-tailed t test (e).