Fig. 5: Als3 inhibits apoptosis.
From: Fungal Als proteins hijack host death effector domains to promote inflammasome signaling
a Purified Als3-His directly interacts with recombinant human full-length caspase-8. Pull-down of human full-length caspase-8 with N-Als3-His or N-Als3S170Y-His. Results are representative of three individual experiments. b Pull-down of Als3-interacting proteins in 293 T lysates using Ni-NTA magnetic beads. Results are representative of three individual experiments. c The susceptibility of Vector, N-Als3, FLAG-E8, or FLAG-MC159 expressing Jurkat cells to apoptosis was determined by incubating cells with the indicated concentration of Fc-FasL (ng ml−1) for 24 h. Data are mean ± s.e.m. from two independent experiments with two or three technical repeats (n = 5). P-values were calculated using two-way ANOVA with Tukey’s post-hoc test.
