Fig. 2: Dynamics of most abundant faecal miRNAs in healthy mice over time.
From: An optimised faecal microRNA sequencing pipeline reveals fibrosis in Trichuris muris infection
a Representation of sample collection workflow and nomenclature of faecal samples for small RNA sequencing from naïve mice and mice infected with a low dose of T. muris. b Heatmap comparing the ranking of the top 20 miRNAs measured by mean normalised counts in the healthy control group at the baseline (I0) to the other naïve groups control groups at day 21 and 35 (N21 and N35). c–f Expression of miRNAs on day 21 and day 35 (N21 and N35) shown as a percentage change from the value at day 0 (I0). miRNAs are clustered into sub-plots based on expression pattern over time. Mean and SEM are plotted. Statistical significance was calculated on the normalised counts for each group by two-way ANOVA with Geisser-greenhouse correction and Tukey’s multiple comparisons test. Significance values displayed are for each time point versus I0. c miRNAs that increase incrementally, d miRNAs that increase at day 21 before decreasing at day 35, e miRNAs that decrease incrementally and f miRNAs that decrease at day 21 before increasing again at day 35. I0 = 7, N21 = 5 and N35 = 5 individuals. * = adj. p < 0.05, ** = adj. p < 0.01. Exact P values can be found in the Source Data file or Supplementary Data 2 (for b).
