Fig. 5: WNT11 knockdown in cancer cells promotes T-cell activation and consequent antitumor immunity through CXCL10/CXCR3 and CCL4/CCR5.

a The expression of cytokines was confirmed using qPCR in MC38 and Panc02 scramble, shWnt11, and shWnt11-shAff3 cells. b–e ELISA was used to measure the secretion of CXCL10 and CCL4 in the supernatants of MC38 and Panc02 scramble, shWnt11, and shWnt11-shAff3 cells. f–h FACS analysis of CD8⁺ T-cell proliferation (CFSE low) in cocultures with MC38 and Panc02 scramble and shWnt11 cells, while CXCR3 and CCR5 were inhibited by AMG487 and maraviroc, respectively, in the coculture system. i–l Cxcl10 and Ccl4 expression were detected using qPCR in MC38 and Panc02 cells with CAMKII knockdown or double knockdown of CAMKII and AFF3. m–o Quantification and representative images of liver weight, number of metastases, and IHC staining of CD8a in an LM mouse model established using MC38 cells treated with a combination of a CXCR3 inhibitor (AMG487) and CCR5 inhibitor (maraviroc). p–r Quantification and representative images of liver weight, number of metastases, and IHC staining of CD8a in an LM mouse model established using Panc02 cells treated with a combination of AMG487 and maraviroc. b–e, g–m, p P-values were calculated using a one-way ANOVA followed by a post hoc Tukey’s multiple comparison test, data are presented as the mean ± SD (n = 3 biological replicates). Source data are provided as a Source Data file.