Fig. 4: The EXPERT strategy enhances editing efficiency with high product purity and low off-target effects.

a Frequencies of intended edits and indels introduced by PE2 and EXPERT at multiple loci. Additional mismatches were introduced in the insertion-type edits. Bars represent the mean of n = 3 independent biological replicates. Data are presented as mean ± s.d. b Statistical analysis of normalized editing frequencies, setting the frequencies induced by PE2 as 1. n = 19 editing from independent experiments shown in (a) and supplementary Fig. 9. Data are presented as mean ± s.d. c Statistical analysis of normalized frequencies of indels, setting the frequencies induced by PE2 as 1. n = 19 editing from independent experiments shown in (a) and supplementary Fig. 9. Data are presented as mean ± s.d. d Frequencies of intended edits, indels, and relative product purity introduced by twinPE and EXPERT at different loci. Bars represent the mean of n = 3 independent biological replicates. Data are presented as mean ± s.d. e Numbers of genome-wide base substitutions. Bars represent the mean of n = 3 independent biological replicates. Data are presented as mean ± s.d. The P value was calculated using a two-tailed t-test, and no adjustments were made for multiple comparisons. f Numbers of genome-wide indels. Bars represent the mean of n = 3 independent biological replicates. Data are presented as mean ± s.d. The P value was calculated using a two-tailed t-test, and no adjustments were made for multiple comparisons. All sequencing data were collected from transfection-positive cells. Source data are provided as a Source Data file.