Fig. 5: EXPERT strategy can enhance efficiency across various PE systems and can be applied to different cell types from multiple species.

a Frequencies of intended edits and indels introduced by PE2max, PE3max, PE4max, PE5max and their corresponding EXPERTmax systems. Additional mismatches were introduced in the insertion-type edits. Bars represent the mean of n = 3 independent biological replicates. Data are presented as mean ± s.d. b Frequencies of intended edits and indels introduced by PE2max and EXPERTmax in K562 cells. Additional mismatches were introduced in the insertion-type edits. Bars represent the mean of n = 3 independent biological replicates. Data are presented as mean ± s.d. c Frequencies of intended edits and indels introduced by PE2max and EXPERTmax in Jurkat cells. Additional mismatches were introduced in the insertion-type edits. Bars represent the mean of n = 3 independent biological replicates. Data are presented as mean ± s.d. d Frequencies of intended edits and indels introduced by PE2max and EXPERTmax in Hela cells. Additional mismatches were introduced in the insertion-type edits. Bars represent the mean of n = 3 independent biological replicates. Data are presented as mean ± s.d. e Frequencies of intended edits, indels, and product purity introduced by PE2max and EXPERTmax in N2a cells. Bars represent the mean of n = 3 independent biological replicates. Data are presented as mean ± s.d. f Frequencies of intended edits, indels, and product purity introduced by PE2max and EXPERTmax in PFF cells. Additional mismatches were introduced in the insertion-type edits. Bars represent the mean of n = 3 independent biological replicates. Data are presented as mean ± s.d. g Schematic diagram of complex mutations in CFTR exon 4. The intended edits that carried four mutations were performed in HEK293T cells using PE2max, PE3max, EXPERTmax, and EXPERTmax + nicking sgRNA, respectively. h Frequencies of intended edits, indels, and product purity introduced by PE2max, PE3max, EXPERTmax, and EXPERTmax + nicking sgRNA, respectively. Bars represent the mean of n = 3 independent biological replicates. Data are presented as mean ± s.d. All sequencing data were collected from transfection-positive cells. Source data are provided as a Source Data file.