Fig. 2: A genome-wide screen to identify cellular inhibitors of split transgene reconstitution. | Nature Communications

Fig. 2: A genome-wide screen to identify cellular inhibitors of split transgene reconstitution.

From: Double-strand break repair pathways differentially affect processing and transduction by dual AAV vectors

Fig. 2

A Screen schematic: a Brunello knock-out Library of U2-OS cells (500x coverage) was treated with 5e3 VG/cell of the HB dual vectors. Cells were collected at 48 hours post transduction, stained with 4-Methylumbelliferyl-beta-D-galactopyranoside (MUG), and MUG negative (NEG) and positive (POS) cells separated by FACS. B Reactome pathway analysis results on the top 5% of genes in the positive population (candidate negative regulators of split transgene reconstitution), sorted by significance. Bubble size indicates the number of genes in each Reactome category. HDR = homology directed repair. C Guide-enrichment score (sgRNAs enriched in the MUG-positive population) sorted in ascending order and plotted as a waterfall plot. Rad51 and BRCA1 are highlighted. D -log10(p-value) of guide enrichment scores sorted and plotted in ascending order. Rad51 and BRCA1 are highlighted. See Methods for analysis details and plotting code.

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