Fig. 5: Rad51 inhibition increases dual vector transduction. | Nature Communications

Fig. 5: Rad51 inhibition increases dual vector transduction.

From: Double-strand break repair pathways differentially affect processing and transduction by dual AAV vectors

Fig. 5

The effects of (A–C) Rad51 or (D–F) DNA-PK inhibition on split transgene reconstitution were tested in U2-OS cells by LacZ activated fluorescence assay (LAFA) 48 hours post-transduction by the dual (or single) vector indicated above each plot. Bars represent the mean of 5 (A–C) or 4 (D–F) biological replicates; individual values are plotted as dots, bars represent SD. A 2-way ANOVA was used, with *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001. G U2-OSlacI-mNeonGreen cells were pretreated with DMSO or B02, transduced with 1e5 VG/cell AAV2/2.lacO.64, and immunostained for Rad51, 53BP1, or BRCA1 as listed at top of panel using an Alexa-647 secondary antibody at 48 hpt. H LacI and 647 foci colocalization (center of LacI focus overlapping with 647 spot) was quantified by high content imaging in biological duplicate and reported as a percentage of total foci imaged with a minimum of 931 foci imaged per data point (bar represents average of individual plotted points). Source data for plots are provided as a Source Data file. DMSO = dimethyl sulfoxide.

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