Fig. 3: Nutrient recycling is independent on Lon protease activity in the live cell.
From: Bacteria encode post-mortem protein catabolism that enables altruistic nutrient recycling
a Plot of the change in cell density for Lon-null cells grown at 37 °C in M9/1% (v/v) glycerol media for 20 hrs in the presence or absence of WT or Lon-null lysates and the indicated Lon WT or mutant protein encoding plasmids (n = 6; mean ± 95% C.I., p values - one-way ANOVA with post hoc Tukey’s multiple comparisons test, F (DFn = 4, DFd = 25) = 44.50). Comparisons for which the Δlon phenotype is complemented (WT vs Lon-null, WT vs Lon-null + protease/ATPase-null Lon, WT vs H2O p < 0.0001; H2O vs WT, H2O vs Lon-null + Lon WT p < 0.0001). Comparison for which the Δlon phenotype is not complemented (Lon-null vs H2O p = 0.1869). b Plot of the change in cell density for Bacillus subtilis grown at 30 °C in M9/1% (v/v) glucose media for 20 hrs in the presence or absence of WT, Lon-null, or B. subtilis lysates and the indicated Lon WT protein-encoding plasmid (n = 10; mean ± 95% C.I., p values - one-way ANOVA with post hoc Tukey’s multiple comparisons test, F (DF = 4, DFd = 45) = 324.1). Comparisons for which the Δlon phenotype is complemented (WT vs Lon-null, WT vs Bacillus subtilis, WT vs H2O p < 0.0001; H2O vs Lon-null + Lon WT p < 0.0001). Comparison for which the Lon-null phenotype is not complemented (Lon-null vs H2O p = 0.9957). Source data are provided as a Source Data file.
