Fig. 4: Nutrient recycling is dependent on post-mortem Lon protease activity.
From: Bacteria encode post-mortem protein catabolism that enables altruistic nutrient recycling
Plots of the change in cell density for E. coli BW25113 grown at 37 °C in M9/1% (v/v) glycerol media for 20 hrs in the presence or absence of lysate derived from E. coli BW25113 WT (WT) or BW25113Δlon (Δlon) and the indicated recombinant Lon proteins. a Wild type and mutant E. coli Lon protease (n = 6; mean ± 95% C.I., p values - one-way ANOVA with post hoc Tukey’s multiple comparisons test, F (DFn = 7, DFd = 40) = 18.81). WT vs Lon-null, WT vs Lon-null + buffer, WT vs protease-null Lon, WT vs H2O p < 0.0001; WT vs protease/ATPase-null Lon p = 0.0010; WT vs ATPase-null Lon p = 0.1349; Lon-null vs H2O p > 0.9999. b Wild type E. coli Lon protease and Thermococcus onnurineus NA1 Lon protease (TonLon) (n = 10; mean ± 95% C.I., p values - one-way ANOVA with post hoc Tukey’s multiple comparisons test, F (DFn = 4, DFd = 45) = 51.51). H2O vs WT + buffer, H2O vs Lon-null + TonLon, H2O vs Lon-null + Lon WT p < 0.0001; H2O vs Lon-null + buffer p = 0.5634. Source data are provided as a Source Data file.
