Fig. 9: efk-1 mutants are sensitive to starvation-induced oxidative stress. | Nature Communications

Fig. 9: efk-1 mutants are sensitive to starvation-induced oxidative stress.

From: Eukaryotic Elongation Factor 2 Kinase EFK-1/eEF2K promotes starvation resistance by preventing oxidative damage in C. elegans

Fig. 9: efk-1 mutants are sensitive to starvation-induced oxidative stress.The alt text for this image may have been generated using AI.

A, B The micrographs show ROS content in freshly hatched and day 6 starved WT and efk-1 mutants with or without 5 mM NAC, as represented by superoxide dyes A dihydroethidium (DHE) and B CellROX Green. N = 3 (total 100 ~ 120 live worms per condition). Data are quantified in Fig. S9A–D. Scale bar, 100 µm. C, D The graphs show L1 starvation survival of WT and efk-1 mutants with or without supplementation of antioxidants C NAC and D VitC at 5 and 2.5 mM, respectively. WT and efk-1 controls shown are of the same experiments. N = 3, error bars represent mean ± SD; for C, **p = 0.0051 untreated efk-1 vs. WT, **p = 0.0076 treated vs. untreated efk-1; for D, **p = 0.0011 untreated efk-1 vs. WT, *p = 0.0488 treated vs. untreated efk-1 (AUC compared using one-way ANOVA with Tukey’s multiple comparisons test). E–H The graphs show L1 starvation survival of WT, E zip-2, F cep-1, G xpa-1 or H xpf-1 mutants with or without supplementation of 5 mM NAC. WT and WT + NAC controls shown are of the same experiments between E, F and G, H. N = 4, error bars represent mean ± SD; for E, **p = 0.0016 untreated zip-2 vs. WT, *p = 0.0181 treated vs. untreated zip-2; for F, ***p = 0.0006 untreated cep-1 vs. WT, **p = 0.0053 treated vs. untreated cep-1; for G, **p = 0.0015 untreated xpa-1 vs. WT, **p = 0.0016 treated vs. untreated xpa-1; for H, **p = 0.0087 untreated xpf-1 vs. WT, **p = 0.0074 treated vs. untreated xpf-1 (AUC compared using one-way ANOVA with Tukey’s multiple comparisons test). I The graph shows larval UV-C survival (y-axis) of WT and efk-1 mutants (black, mock treatment; red, 10 J/m2 UVC) after pretreatment with starvation of up to 9 days (x-axis). N = 3, error bars represent mean ± SD (AUC of mock treatment vs. UV-C compared using one-way ANOVA with Tukey’s multiple comparisons test). WT, wild-type; ns, not significant; ROS, reactive oxygen species; NAC, N-acetylcysteine; VitC, Vitamin C (ascorbic acid); AUC, area under the curve. Source data are provided as a Source Data file.

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